Welcome to the Overlap Extension Primers Calculator, the tool Rick Sanchez would invent if he was a geneticist and not too busy dodging the Galactic Federation! Sick of tedious experiment planning? Throw in your main sequence and let's get schwifty with DNA splicing! This tool whips up primer pairs to seamlessly fuse sequences—no scars, all science. Let's make your DNA work wonders!
All sequences are generated in a 5'->3' direction. mT is calculated with Wallace Rule.